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Y2H cDNA library construction and screening procedure. A) The conventional procedure for cDNA library generation. B) Using the cDNA library in Y2H screens to identify protein–protein interactions. C) Using the cDNA library to screen for rescue genes involved in DNA repair. D) Screening randomly selected plasmids from the cDNA library to screen random samples from the library “Created with Biorender.com .”.

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

doi: 10.1016/j.jgeb.2024.100427

Figure Lengend Snippet: Y2H cDNA library construction and screening procedure. A) The conventional procedure for cDNA library generation. B) Using the cDNA library in Y2H screens to identify protein–protein interactions. C) Using the cDNA library to screen for rescue genes involved in DNA repair. D) Screening randomly selected plasmids from the cDNA library to screen random samples from the library “Created with Biorender.com .”.

Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

Techniques: cDNA Library Assay, Protein-Protein interactions

The 5′ end truncated cDNAs identified, bp; base pairs.

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

doi: 10.1016/j.jgeb.2024.100427

Figure Lengend Snippet: The 5′ end truncated cDNAs identified, bp; base pairs.

Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

Techniques: Variant Assay, Binding Assay, RNA Binding Assay

mRNA priming in cDNA libraries. A) Normal priming through reverse transcriptase elongation of cDNA after oligo (dT) binding to true poly-A tails. B) Internal priming that leads to the generation of truncated transcripts due to internal A-rich regions and reverse transcriptase stalling. “Created with Biorender.com .”.

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

doi: 10.1016/j.jgeb.2024.100427

Figure Lengend Snippet: mRNA priming in cDNA libraries. A) Normal priming through reverse transcriptase elongation of cDNA after oligo (dT) binding to true poly-A tails. B) Internal priming that leads to the generation of truncated transcripts due to internal A-rich regions and reverse transcriptase stalling. “Created with Biorender.com .”.

Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

Techniques: Reverse Transcription, Binding Assay

Mitochondrial hits status and their quantification.

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

doi: 10.1016/j.jgeb.2024.100427

Figure Lengend Snippet: Mitochondrial hits status and their quantification.

Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

Techniques: